Fluidics Setup
More about pumps here.
More about tubing and connectors here.
Fit tubing to your application
If you are doing ePACE in the min-eVOLVER, check the experiment for fluidic routing
Make sure it's easy to get both tube ends into a beaker for sterilization
Alternatively you can connect an additional line to short tubing for easy sterilization
To-Vial Tubing Method (Right Port of All Pumps)
Start with tubing that is longer than you think you will need - you can trim it later easily
Put eVOLVER vials with caps in the smart sleeves
Estimate the tubing lengths for the to-vial pump tubing
Efflux (Pumps 1 + 2 Right Port)
Use clear blue 1/16" ID tubing
Can be relatively short because it connects just to the vial
Waste (Pumps 1 + 2 Left Port)
Should reach from left port of bottom pumps into waste carboy
Media influx + Inducers (Pumps 3 - 6)
Use clear 1/16" ID tubing
Left pump port should be long enough to reach media / inducers easily
Right pump port can be relatively short because it connects just to the vial
