Overview

An essential component that screws on top of the glass eVOLVER vial. Connects to eVOLVER fluidics via luer lock.

Versions

4 Port Universal [Needle or Luer Lock]

Overview

Option 1: [Recommended] Can be easily constructed with luer locks

Option 2: [Not Recommended] Can be used with needles like old caps, however this opens you up to preventable overflows as the old caps did

Advantages

1. [ enabled] - prevent vial overflows via air tight seal to vial

2. Reusable connectors (no need to use needles) - connect tubing directly to luer lock on cap

3. Easy assembly with few additional parts

Assembly

GitHub

5 and 7 Port [Nylon Tube Only]

Overview

Use a honeycomb pattern for the ports to cram more ports onto the cap.

Advantages

1. [ enabled] - prevent vial overflows via air tight seal to vial

2. Reusable connectors (no need to use needles) - connect tubing directly to luer lock on cap

3. Up to 5 or 7 ports per cap (plus sampling port) - other caps only have 4 ports

Assembly

GitHub

[Old] Needle-Only

GitHub

Overview

This is the cap that is currently given by default from FynchBio as of 25/01/15.

Requires needles, so cannot have a full seal on the vial. Therefore, should be used with caution because of overflow risk.

Assembly

Must be assembled with needles at correct heights before each run.

Materials

• 3D printed vial cap with ports for nylon tubing

  • the .stl file from GitHub

  • Order from

    • Service = SLS; material = nylon PA-12

    • Get "vapor smoothed" for better sealing

• (1/8" OD)

• flexible silicone rubber tubing (3/32" ID (inner diameter))

• flexible silicone rubber tubing (3/32" ID (inner diameter))

• Five 3/32" female barb to luer

• Mixing tray (here a small weighboat)

• Disposable applicator (here a wooden stir stick; pipette tip also works)

• [Optional - for color coding ports] flexible silicone rubber tubing (3/32" ID (inner diameter))

• [Optional - for color coding ports] flexible silicone rubber tubing (3/32" ID (inner diameter))

• [Optional] 3D printed (SLS, nylon PA-12, vapor smoothed) for in-vial aeration

Design

1. Choose the minimum number of ports necessary

   1. You will need to block up ports that are unused if you want to do emergency efflux

   2. Therefore, choose the cap with the minimum number of ports that you need

Construction Protocol

0. Mark fluid lines on a glass eVOLVER vial (see above image)

1. This will be used to set the efflux tube height, which sets the volume in the eVOLVER vial.

2. Tape an eVOLVER vial

3. Using a serological pipette, measure water into the vial in increments of 5 mL

1. Cut Nylon Tubing to Various Lengths Needed

1. Cut the necessary components of the hard semi-clear white nylon tubing (1/8" OD). Refer to the table below for lengths.

The table below has the necessary components for the construction of one cap.

1. Cut the necessary components of the flexible nylon tubing. Refer to the table below for lengths.

The table below has the necessary components for the construction of one cap.

2. Assemble Cap

Steps

1. You may need to drill out the ports in the 3D printed vial cap if powder is blocking them.

2. Insert the hard semi-clear white nylon tubing (1/8" OD) into each corresponding port in the cap as per the image below.

1. Screw the cap into the glass vial with measuring tape on the side and ensure that:

• The 2 influx tubes ARE touching the edge of the vial. They should be staggered such that one is higher than the other.

• [Bubbler Only] Bubbler tube is at the 15mL line on the vial.

• [Bubbler Only] Bubbler IS NOT touching the edges of the vial and is facing the sampling port (rotate and adjust as necessary to ensure this).

1. Follow the epoxy directions to mix up a small amount of epoxy in a disposable dish. Use even pressure to get even amounts of resin and hardener. Use a toothpick or other similarly sized applicator to put the minimum amount of epoxy around the outside area of the cap where the ports and the hard nylon tubing connect, ensuring to spread the epoxy in between any gaps.

2. Set assembled caps upright (with a vial rack or spare vials) so that epoxy doesn't drip into the sampling port as it cures.

3. Set cap on its side to cure overnight.

Materials

• 3D printed (SLS, nylon PA-12) vial cap

  • the .stl file from GitHub

  • Order from

  • Get these "vapor smoothed" for better sealing

  • Get white nylon PA12 (the default black will leach dye into your media)

  • Other default settings are fine

• 4X - 3/32" female white nylon

  • Buy at least 4 x number of caps + extra

  • Nylon required to survive autoclaving (expansion differential between normally used polypropylene and nylon 3D printed vial causes epoxy to separate)

• (1/8" OD)

• [Required for overflow protection]

• [Required for overflow protection]

Construction Protocol

1. [Optional] Mark fluid lines on a glass eVOLVER vial (see below image)

   1. This will be used to set the efflux tube height, which sets the volume in the eVOLVER vial.

   2. Tape an eVOLVER vial

1. Use gloves when working with epoxy

2. Put epoxy into mixing tray and mix

   1. You don't need much because epoxy will likely set before you finish all vials

1. Apply epoxy to barb of luer connector

   1. Be generous, but avoid the opening in the barb

   2. If you do get epoxy in the opening, blow it out or pick it out with a paperclip

1. Press the connector into a port in the top of the cap, and twist a few times to spread epoxy

1. Repeat steps 4 and 5 for the other ports

   1. When epoxy starts to solidify, mix more (in a new tray)

2. Wait for at least an hour for epoxy to set somewhat

1. Repeat steps 8 and 9 for all caps

2. Allow epoxy to set 24 hours before using