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Operating Millifluidics

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Always use less than 10 psi inputs into the millifluidics and pressure regulator to avoid breaking them

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Never use ethanol to sterilize millifluidics as it breaks them. Use bleach to sterilize and flush it with sterile water.

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Set Up

Plug in 5V power supply and 5V fan

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Changing Pressure Settings

Option 1: Manually change via Arduino (hard coded)

  • Follow the Arduino Software Installation

  • Unplug the SAMD21 from the pressure regulator before uploading code (otherwise there might not be enough power to the SAMD21 from the USB)

  • Upload modified pressure code to SAMD21

Option 2: Add a 'pres' command to the evolver conf.yml and modify conf.yml

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Fluidic Lines

Connect a 8-10 psi air input into the input port (only port on its own in 1.0 design)

Connect regulated line to both sensor (below) and controlled output (white, above) as shown below:

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Set Up Solenoid Control

Solenoid Bank Diagram (facing holes)

  • The solenoids should be normally open and connected to the 8 psi air source. ie Vacuum when closed

  • Left = 8 psi

  • Right = Vacuum

Connect solenoid ribbon cable to slot 5 on the fluidics box

Turn on eVOLVER server and fluidics box

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Check that the solenoid bank is correctly set

Run ipp_cal.py on the server:

  • Use command:

    • python3 ipp_cal.py <frequency (hz)> <seconds to run>

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Set Up IPPs

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IPPs need to be and before use.

It is recommended to calibrate using a pressurized input line (ie a 1.5 psi bottle). Always use that same pressure on the fluidic input.

  • Hook IPPs up to a pressurized input bottle for consistency

  • Make sure that IPPs are operating as expected and producing the expected flow rate

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If running a trial experiment with just water, you can put food dye in to the inducer to visualize how much is injected into vials

Middle = 8 different solenoids, groups of 3 are IPP controls

For example: python3 ipp_cal.py 1 100

Pressure Regulator
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guide
ssh to server
constructed
calibrated