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Design

Eagle

• Using Eagle to drag and drop elements + make layers

• Problem with Eagle

Zack defines width of the pumps, but doesn't worry about depth

Width of channel / valves

• Too small = hard to align + valves will no longer stay closed

• Larger is more robust

Barb spacing

• Want enough space between each barb for tool

• Far enough from the edge

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Set Up

Plug in 5V power supply and 5V fan

Changing Pressure Settings

Option 1: Manually change via Arduino (hard coded)

• Follow the Arduino Software Installation

• Unplug the SAMD21 from the pressure regulator before uploading code (otherwise there might not be enough power to the SAMD21 from the USB)

• Upload modified pressure code to SAMD21

Option 2: Add a 'pres' command to the evolver conf.yml and modify conf.yml

Fluidic Lines

Connect a 8-10 psi air input into the input port (only port on its own in 1.0 design)

Connect regulated line to both sensor (below) and controlled output (white, above) as shown below:

Set Up Solenoid Control

Solenoid Bank Diagram (facing holes)

• The solenoids should be normally open and connected to the 8 psi air source. ie Vacuum when closed

• Left = 8 psi

• Right = Vacuum

Connect solenoid ribbon cable to slot 5 on the fluidics box

Turn on eVOLVER server and fluidics box

Check that the solenoid bank is correctly set

Run ipp_cal.py on the server:

• Use command:

  • python3 ipp_cal.py <frequency (hz)> <seconds to run>

Set Up IPPs

• Hook IPPs up to a pressurized input bottle for consistency

• Make sure that IPPs are operating as expected and producing the expected flow rate

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Protocol

Control Layer (1/4" acrylic)

Cut outlines first

• 0.1pt

• 3 copies

Keep full stock in place + outline on computer

• Acts as a frame for the device

• Remove board from stock with tweezers

Add adhesive

Put adhesive covered outline back in place on stock

On the computer

• Align control vector cuts (ports) to the outlines

• Delete outlines

• Print (3 copies for 1/4")

Keep board in place

Rastering control channel

• Undo deletion of outline, delete previous vector cuts

• Align raster outline to old outline + delete outline

• Load control_channel 2 in advanced settings

Remove board + stock from laser cutter

Flow Layer (1/8" acrylic)

Cut adhesive sheet

• Copy/paste in design with scooped out region around bridge

• Cut adhesive out first

• This way we don't have to move the acrylic stock later to put adhesive on the laser cutter

Cutting acrylic

• Delete scoops, we just want the outlines

• Print vector cut for outline

• Refocus laser cutter to 1/8th inch + cut

Plasma clean acrylic

Put adhesive on

Rastering acrylic

• Place device back in stock on laser cutter

• Copy/paste raster of flow route

• Remove outline

Assembly

Tap control board

• 2 turns one way, little turn the other way

• Use a countersink to remove burrs around the holes

  • A couple of turns

Remove backing from all acrylic (helps with alignment later)

Silicone

• Sam from Densmore lab bought a big roll

• Some in drawer under cutting station

• Cut larger size than device

Apply silicone to control board

• Align and press onto control board

• Take off plastic from other side of silicone

• Wrap in paper towel

Punch holes in silicone

• Use biopsy punch (green on shelf above 3D printers) to make holes silicone for flow

• Don't need to do the control

• Put pressure and turn

Clean silicone + adhere to flow layer

• Use piece of packing tape to remove dust

• Plasma clean silicone 30 seconds

• Align flow and control

Put loctite on barbs + screw in to board

• Barbs are in Dan's drawer

Subprotocols

Laser Cutter

Different colors of line produce different depth / intensity of cut

• Use black for everything

Make all lines hairline outline

• Otherwise it won't cut!

Open up a new page

• Paste whatever you're cutting into this

• This will be the correct size of bed

Printing

USB Bullshit

• Open up printers / devices

• Have to reconnect USB multiple times for it to be recognized

1. Open up print

2. Select correct printer you figured out

3. Load the correct settings

On the Laser Cutter

Make sure it's on / connected

Focus first using metal thing attached to laser

Run the job with lid open to double check it's doing what you hope

Run the job

Plasma Cleaning

Plasma treat surfaces (except adhesive)

• Take off one side of acrylic backing

• Vacuum knob arrow left

• Metal valve tight, then quarter turn loose

Adhesive

Adhesive is in a box above 3D printers

Cut adhesive to larger size than acrylic

• Remove adhesive backing and place face up on surface

• Remove acrylic from plasma cleaner and firmly press clean side onto adhesive

• Squeegee the bubbles out as much as you can (and away from where the channels will be)

Page under construction

Page under construction