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min-eVOLVER Construction

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Purchasing a Unit

min-eVOLVERs may not be purchased as a product at this time. Instead min-eVOLVER parts must be acquired individually and assembled.

min-eVOLVER parts laid out

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Requirements for Construction

  1. Soldering iron + solder - pumps will need to be soldered in place

  2. Ability to through-hole solder

    1. Can be self-taught quickly through YouTube videos

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Questions About min-eVOLVER Construction or Hardware?

Ask questions on the forum .

Screw drivers

  1. Phillips #1 (short handle)

  2. Flat head #1.5 or smaller

  • Ideally, some kind of clamp, vice grip, etc.

    1. For making ribbon cables to connect the electronics

    2. If nothing else, a hammer or pair of pliers will suffice

  • herearrow-up-right

    Construction Protocol

    It may be helpful to have the parts page open as well, so you can identify what you need for each step.

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    Make Ribbon Cables

    (A) Ribbon cable connector placed at 5" with both knobs on the same side (arrows). (B) Align the ribbon cable connector with the end of the cable. (C) Connector is placed in center of clamp. (D) Completed ribbon cable after connector is crimped on.
    1. Measure ribbon cables and mark length (figure above A)

      1. 5" ribbon cable for pump board to min-eVOLVER PCB connection

      2. Two 4" ribbon cables for smart sleeve PCB to min-eVOLVER PCB connection

    2. Cut ribbon cables to size using scissors or razor.

    3. Open the connector and align to the end of the ribbon cable (figure above B)

      1. The connectors should both have the knob on the same side (figure above A, red arrows)

      2. You can apply pressure to the connector so that it stays in place as you work with it

    4. Crimp on ribbon cable connectors (one on each side of cable)

      1. Be careful not to break the connectors, they're fragile!

      2. The easiest way crimp these connectors is to use a clamp or vice (figure above C), unless you have a specialized tool

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    Prepare case

    1. Unscrew the 3 screws on the top of the case (figure below, left)

    2. Unscrew the 2 screws on the bottom (figure below, right) and unscrew the right middle rubber foot (yellow arrow)

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    Construct Pump Board

    Solder 2x8 ribbon cable connector into pump PCB, screw pumps into front of case, and solder pumps into pump PCB.

    Use the below video:

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    Construct min-eVOLVER PCB

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    Surface mount soldering of the min-eVOLVER PCB is outside of the scope of this guide. It is assumed that you have an already assembled min-eVOLVER PCB.

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    Solder SAMD21 Arduino

    1. Place the 2x12-pin header on a workbench with the flat (non conical) side of the pins up (figure below A). Place the SAMD21 board on top of it

      1. You can also carefully plug the SAMD21 into a breadboard if you want to avoid it moving

    2. Carefully solder the SAMD21 board pins.

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    Assemble min-eVOLVER PCB

    1. Press the SAMD21 board into the min-eVOLVER PCB until you feel a click and the SAMD21 sits mostly flush

      1. Micro-USB port should be on edge of board

      2. Carefully align the pins with the sockets

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    OD 90 vs OD 135

    1. OD90 = OD LED and photodiode at 90 degrees from eachother

    2. OD135 = OD LED and photodiode at 135 degrees from eachother

    3. By default, we choose to use OD90 for our vials, which is typically better at measuring higher ODs > 0.4 and is what most people want

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    Assemble min-eVOLVER PCB and pumps with the case

    1. Place min-eVOLVER PCB on standoffs. Screw 1/4" screws into the front left and back right corners (figure A below)

    2. Plug the 5" ribbon cable in to the pumps and to the ribbon cable port to the left of the SAMD21 (figure B below)

    3. Close the case and replace the 5 screws and rubber foot that you had removed previously (figure C below)

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    Construct eVOLVER smart sleeves and screw in to case

    1. If your smart sleeves came disassembled:

      1. See general smart sleeve construction guide

      2. Firmly push the OD LED and photodiodes in to the smart sleeve

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    Flash the min-eVOLVER with Arduino code

    1. Plug the min-eVOLVER into your computer via the micro-USB cable

      1. Do not plug in the 12V power supply

    2. Download the min-eVOLVER Arduino

    Parts

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    Parts Spreadsheet

    Below is a spreadsheet containing all parts required to make a min-eVOLVER.

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    Place the connector in the center of the clamp (making sure it remains in the right location)

  • Tighten clamp until sides of connector are flush and it is full closed (figure above D)

  • You can also use pliers, but this is more annoying / prone to breaking the connectors

  • Avoid adding too much solder so that it globs onto the small electronic components!

  • Start with the four corners to lock it flat in place on the header

  • Solder all pins

    1. If you want to be extra careful, only solder half the pins on a side before switching to the other side (to avoid overheating the board)

  • Make sure that each pin is individual and no solder has reached the electronic components on the board surface

  • [Recommended] Strengthen SAMD21 Board USB

    1. The micro-USB port on the SAMD21 easily pops off when you move the ribbon cable

    2. You will have a useless board and it will be necessary to get a new one

    3. Therefore, you should add to the perimeter of the connector in the (locations shown in the figure below C)

    4. Follow directions on the epoxy label for use

    5. Use gloves when working with epoxy!

    6. A weigh boat and pipette tip work for mixing and application

    7. Important - Avoid putting epoxy into the holes in the micro-USB connector

    8. Otherwise, be generous with the epoxy, it's ok to get it on the electronics

  • Slide the shorting jumpers onto the right two pins for each vial (see figure above D)

    You can also place the jumper across the left two pins for each vial, meaning you will register OD135.

  • You will need to make different OD calibrations for OD135 and OD90

  • Do not use the acrylic base plate (clear plastic below the computer fan)

  • If your smart sleeves came assembled:

    1. Unscrew them enough to remove the acrylic base plate. Keep the fan spacer on (figure A below)

    2. We will also not use the acrylic base plate and instead directly screw our smart sleeves to the case

  • Screw the smart sleeves into the min-eVOLVER (figure B below)

    1. It can be easier to tighten each screw a little at a time to avoid misalignment

    2. Do not over tighten screws, go until smart sleeves are firmly in place

  • Plug 3.5" ribbon cables into back of smart sleeves and to the min-eVOLVER (figure C below)

  • Place vial cover over vials (figure D below)

  • Follow the Arduino Software Installation guide

  • Upload the min-eVOLVER Arduino code onto the device

  • here
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    (Left) Remove 3 screws from the top of the case. (Right) Unscrew the 2 screws on the bottom and twist off the right middle rubber foot (yellow arrow).
    (A) The SAMD21 board and the 2x12-pin header with flat side of the 24-pins up. (B) SAMD21 board partially soldered, starting from the pins in the corners. (C) Locations for epoxy (cyan) avoiding openings in the micro-USB connector. (D) Placing the jumpers on the right two selector pins for each vial. (E) The assembled min-eVOLVER PCB.
    (A) min-eVOLVER on standoffs inside case and screwed down with 1/4" screws. (B) Demonstrating how the ribbon cable connecting pumps and min-eVOLVER PCB should be plugged in. (C) Assembled min-eVOLVER PCB, pumps, and case.
    (A) Screws unscrewed enough to remove the base plate, which is sitting next to the vial. Fan spacer on the bottom is left on. (B) Smart sleeves are screwed in to the case. (C) Smart sleeves are connected to the case via 3.5" ribbon cables. (D) Vial cover is placed over vials.

    Read the notes below the spreadsheet as well.

  • Don't miss the "Consumables / General" tab. These items also need to be acquired before running experiments.

  • The parts necessary for the min-eVOLVER main unit. Use the spreadsheet above to discern what each item is.

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    min-eVOLVER PCB

    If you are making a min-eVOLVER Control Board PCB from scratch you have two options (as of 11/20/2024):

    1. [Recommended] Order an assembled PCB from PCBWayarrow-up-right (guide below) or another manufacturer

    2. Buy the parts needed from Digikey, order the PCBarrow-up-right from PCBWayarrow-up-right or another manufacturer, and solder the board yourself

      1. NOT recommended for anyone not already familiar with surface mount soldering.

      2. An interactive bill of materials (for part placement) can be found . Download it and open it into your browser.

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    Ordering an Assembled min-eVOLVER PCB from PCBway

    1. Make an account on PCBWayarrow-up-right.

    2. On the homepage, scroll down and click on PCB assembly

    1. Choose the following:

      1. Turnkey, Single pieces, Both sides, fill out the quantity you want, select No "Select PCBway's PCB Order #", uncheck PCB Specifications, and click the green 'Calculate' button.

    1. Download the following files from thisarrow-up-right folder, upload to PCBway, and click "Submit the File Now":

      1. Gerber files: min-eV-gerbers_240731.ziparrow-up-right

      2. Parts List (BOM): BOM_PCBWay.xlsxarrow-up-right

      3. Centroid File:

    1. Wait for PCBway to review your order (1-2 business days) before paying

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    Pump PCB

    Should be ordered from PCBWayarrow-up-right or another manufacturer. You can find the .zip of the gerber files herearrow-up-right. Assembly is not needed.

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    min-eVOLVER Case

    Options:

    1. We use Protocasearrow-up-right to manufacture the cases

      1. Request a quote for the number of cases you require by emailing Jenn Hurd (jhurd@protocase.com)

      2. Mention you want to order the previously ordered name and number below:

        1. Boston University min-eV Case Rev2.1 T120324039-205634-1

    2. For other manufacturers, give them the .x_t, .easm, and .step files found

      1. Request sheet metal construction out of 18 gauge steel, ideally a powder coated exterior

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    12V Power Supply

    Several 12V power supplies were tested and were selected for reliability and low noise. While most 12V power supplies should work well in the short term, long term usage may vary. Use a different power supply at your own risk.

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    Vial Cover

    NOT AN OPTIONAL COMPONENT - Protects OD photodiodes from environmental light, which will completely change your OD calibrations. Also protects smart sleeves from spills and splashes.

    3D print the latest vial cover to allow for more consistent OD readings. Choose the cheapest black FDM printing on hubs.com, shapeways.com, or a local 3D printing shop and give them the .stl file in the github repository.

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    eVOLVER Smart Sleeves

    May be purchased from Fynch Bio. If interested in making your own see the main parts sheet and follow this assembly guide. This is not recommended for its added complication (you will need to machine the aluminum tube for example), but will save money.

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    Consumables / General

    Listed in the "Consumables / General" sheet of the above spreadsheet

    • Anything not listed as (Optional) is required for a full experiment.

    • You need a thermometer for calibrating temperature - we suggest thisarrow-up-right one.

    • If you plan to run multiple min-eVOLVERs from a computer, you will need to have a USB port available for each one. You can use a USB splitter dongle to accomplish this. Example herearrow-up-right.

    epoxyarrow-up-right
    herearrow-up-right
    PCBWAY-min-eV-positions-centroid.csvarrow-up-right
    herearrow-up-right