From Fynch Bio:
eVOLVER base
Fluidics box
2X fast pump racks
[Optional] Additional slow pump rack for inducers
Dedicated computer for experimentation
Mac strongly recommended for ease of setup and reliability
The following are different from the base eVOLVER :
Additional fluidics
We pause stirring and phototroph growth lights when we take OD. Without this, OD would be nonfunctional. Stirring causes bubbles be pulled downwards and the growth lights completely saturate the OD sensor.
to calibration conf.yml before doing calibration
Because this will give us data every 20 seconds and greatly speed up calibration
Settings during calibration should mimic the experiment therefore:
Gas mixer (or tank of premixed gas with regulators)
Vials (recommended to get parts for at least two sets)
Bubbler (plugs in to cap)
Long thin stir bars - required otherwise bubbles will stick and throw off OD readings
Bubbler cleaning apparatus (recommended for saving time)
Phototroph growth lights off
Stirring off
Vial setup for calibration (also mimic the experiment)
Prepare standards in caps with a bubbler
Use long stir bars
When moving standards, give a swirl once every other time
This is to make sure cells don’t settle and change the OD
Finally, switch back to experiment conf.yml when ready for experiment