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Starting an Experiment

Highly recommended:

  2. Run an experiment with water before doing so with cells, especially if you change the code

Questions?

Setup

    1. Instead of the GUI, use send_command.py

    2. The slow pumps (pink) will need to be run for >200 seconds to fully flush the lines

You will have one command line window for each server and each experiment running. It can also be helpful to have a separate window for send_commands.py

For example for two min-eVOLVER experiments:

  • 2 server windows (server environment)

  • 2 experiment windows (dpu environment)

  • 1send_commands.py window (dpu environment)

    1. Use send_command.py to fill the vials to appropriate volumes

Do not inoculate cells until after starting the experiment if you want accurate readings. There is innate variability in the OD of the vials (among other things) and we will blank to your vials during experiment start.

  1. Use send_command.py to set to correct temperature

    1. A calibrated temperature command is in your calibration file temperature_calibration.xlsx

    2. Temperature affects OD readings and we need a good initial OD to act as a "blank"

  2. Allow min-eVOLVER to come to temperature

Running the Experiment

  1. If running more than one min-eVOLVER at a time: you need a separate experiment folder and command line window for each min-eVOLVER (and different port number)

  2. Start the experiment using the command:

    1. python3 eVOLVER.py -i localhost:<your_min-eV_port>

    2. python3 eVOLVER.py -i localhost:5555

Ending an Experiment