If you are new to command line / bash, you can start here!
Reasons you might use command line experiment start:
Doing a custom experiment
Unable to install GUI or getting errors you aren't able to fix easily (post on the forum first)
Set your computer to never fall asleep to prevent experiments from being halted.
In a command line window, enter the dpu virtual environment.
Navigate to the DPU directory
Use this command: cd <path_to_dpu>
You can graph your experiment using the graphing tool .
the experiment
Data is in the
Enter the command:
On Mac OS:
source dpu-env/bin/activate
On Windows PowerShell:
dpu-env\Scripts\Activate.ps1
Navigate to your experiment folder
This should be in dpu/experiment
Copy the template folder and rename it for each experiment
Navigate into your new experiment folder
Alter settings in custom_script.py
EVOLVER_PORT = set correct IP of the eVOLVER
Set initial temp and stir settings
custom_script.py has basic settings for running chemostat and turbidostat experiments, but you can also customize. To learn more click .
Start the experiment using the command:
python3 eVOLVER.py -i <your_evolver's_IP_address>
python3 eVOLVER.py -i 192.168.1.9
Temperature should be fully equilibrated before this
DO NOT add cells before blanking, whatever OD value the cells + media have will be subtracted from future measurements
Stop the experiment using the keyboard shortcut control+C
One control+C pauses the experiment from recording data
Two control+C stops the experiment
To change parameters mid-experiment or restart from blank data files:
Stop the experiment fully
Change parameters in custom_script.py
Restart the experiment
Continue to keep your data
Overwrite to make new blank data files and throw away your old experiment. NOTE: you will lose your OD blank and will need to make a new one (a hassle if you have already inoculated with cells)
You can run experiments on more than one eVOLVER at a time
Create a new experiment folder (different custom_script.py) and change IP
Follow this procedure in an additional command line window