Set up probe in vial using calibration cap

Alter calibration_vals in calibrate_light.py

Start logger

• Turn on via mini-USB power cable plug on top

• Choose 500hz sampling rate

• Press "START"

• New file > Label with vial number > OK > Water probe

• Should be changing values now

Run calibrate_light.py

• python3 calibrate_light.py --ip <evolver_IP> --vial <vial_num> --lights <space_separated_list_of_lights>

• <space_separated_list_of_lights> refers to which light types you want to calibrate (light1, light2, light3)

• Sets eVOLVER light for that vial to each value

• Separates values in the LI-1500 log by turning off the light

Press the START/STOP button to stop logging

Repeat calibration for each vial, moving the light probe and glass vial

1. The vial number in the file name should increase automatically

2. You can overwrite logger files where you mess up

Copy the light_cal_template folder in /dpu/calibrations/ and rename with the name of this light calibration

Plug logger in to the computer via USB

Transfer files over to folder you made

Run analyze_light_cal.ipynb

1. Change the constants in the Jupyter Notebook

2. The fit_name should reflect which light you are calibrating

Transfer your final calibration file to your experiment template folder

The calibration file should be named light{}_cal.txt where {} is replaced with the light number to be recognized by the eVOLVER DPU script

Gas mixer (or tank of premixed gas with regulators)

Vials (recommended to get parts for at least two sets)

1. 5-port nylon tube cap

2. Bubbler (plugs in to cap)

3. Long thin stir bars - required otherwise bubbles will stick and throw off OD readings

Bubbler cleaning apparatus (recommended for saving time)

Light meter and probe

1. Our light meter is the LI-COR LI-1500

2. Our light probe is the Walz US-SQS/L omnidirectional light probe

Stirring off

Vial setup for calibration (also mimic the experiment)

1. Prepare standards in caps with a bubbler

2. Use long stir bars

When moving standards, give a swirl once every other time

1. This is to make sure cells don’t settle and change the OD

Finally, switch back to experiment conf.yml when ready for experiment