Purpose

The min-eVOLVER was originally conceived of as a minimal apparatus to conduct phage-assisted continuous evolution (PACE). Thus it has two bioreactors (eVOLVER Smart Sleeves): one for a "host cell reservoir" and one for a "lagoon". It also has six pumps: four for bulk media in and out of the two Smart Sleeves and two for fine control over inducers into the lagoon.

However, base min-eVOLVER can be used for most continuous culture scenarios, such as turbidostat and chemostat. It can also be readily adapted to new experiments.

Differences from Main eVOLVER

Software-Hardware Architecture

The software architecture of the min-eVOLVER is similar to the original eVOLVER, with the exceptions that:

• The Raspberry Pi server has been replaced with a server run on the connected laptop

• A single Arduino SAMD21 actuates all functions and collects vial data through the min-eVOLVER board

Maximum Power Draw

• Maximum current draw* = 2 Amps

• Power supply voltage = 12V

• Maximum power draw = 24W

*Maximum current draw was measured from a bench-top power supply with the heaters fully on, all pumps running, and stirring on. Instantaneous spikes in current could be higher when pumps and stirring start up

Overview

The min-eVOLVER is a minimal, mini version of the main eVOLVER. It runs in the same software ecosystem with minimal changes to the code. However, the min-eVOLVER controls two eVOLVER Smart Sleeves and six pumps, making it simpler, smaller, and less costly.

Questions or Comments?

If you have questions not answered by the wiki please ask in the relevant min-eVOLVER category on the forum.

Purchasing a Unit

min-eVOLVERs may not be purchased as a product at this time. Instead min-eVOLVER parts must be acquired individually and assembled.

Requirements for Construction

1. Soldering iron + solder - pumps will need to be soldered in place

2. Ability to through-hole solder

   1. Can be self-taught quickly through YouTube videos

3. Screw drivers

   1. Phillips #1 (short handle)

   2. Flat head #1.5 or smaller

4. Ideally, some kind of clamp, vice grip, etc.

   1. For making ribbon cables to connect the electronics

   2. If nothing else, a hammer or pair of pliers will suffice

Questions About min-eVOLVER Construction or Hardware?