Setup Phototroph eVOLVER
Base eVOLVER Requirements
Buy an eVOLVER base
Phototroph eVOLVER Hardware Setup
The following are different from the base eVOLVER setup:
Additional fluidics
Bubbling splitter
[Optional] Additional slow pump rack for inducers
Vials (recommended to get parts for at least two sets)
Bubbler (plugs in to cap)
Long thin stir bars - required otherwise bubbles will stick and throw off OD readings
Bubbler cleaning apparatus (recommended for saving time)
OD Considerations
We pause stirring and phototroph growth lights when we take OD. Without this, OD would be nonfunctional. Stirring causes bubbles be pulled downwards and the growth lights completely saturate the OD sensor.
Additional Calibration Procedures
Switch to calibration conf.yml before doing calibration
Because this will give us data every 20 seconds and greatly speed up calibration
Settings during calibration should mimic the experiment therefore:
Phototroph growth lights off
Stirring off
Vial setup for calibration (also mimic the experiment)
Prepare standards in caps with a bubbler
Use long stir bars
When moving standards, give a swirl once every other time
This is to make sure cells don’t settle and change the OD
Finally, switch back to experiment conf.yml when ready for experiment
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