# Calibrations

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Before beginning calibrations all [Software Installation](https://khalil-lab.gitbook.io/evolver/getting-started/software-installation) and [Network Configuration](https://khalil-lab.gitbook.io/evolver/getting-started/configuring-computer-and-networking) should be complete.
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## About

In order to appropriately interpret data from and actuate physical elements connected to the eVOLVER framework, a relationship must be established between the electrical signals generated by attached sensors and the physical phenomenon they are measuring. This is done by manually measuring values on a controlled set of data via some gold-standard assay or measurement device and comparing these data to the eVOLVER generated data. By doing this across a range of different values, a function can be fit to this relationship to allow eVOLVER to interpret data accurately.

For more information see [here](https://khalil-lab.gitbook.io/evolver/software/dpu/calibration).

## Procedure

Calibrations should proceed in the following order:

1. [Temperature Calibration](https://khalil-lab.gitbook.io/evolver/getting-started/calibrations/temperature-calibration)
2. [Optical Density Calibration](https://khalil-lab.gitbook.io/evolver/getting-started/calibrations/optical-density-calibration)
3. [Pump Calibration](https://khalil-lab.gitbook.io/evolver/getting-started/calibrations/pump-calibration)

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If the GUI fails to calibrate you can try to use [calibrate.py](https://khalil-lab.gitbook.io/evolver/getting-started/calibrations/manual-calibration-calibrate.py) manually.
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