> For the complete documentation index, see [llms.txt](https://khalil-lab.gitbook.io/evolver/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://khalil-lab.gitbook.io/evolver/getting-started/calibrations.md).

# Calibrations

{% hint style="warning" %}
Before beginning calibrations all [Software Installation](/evolver/getting-started/software-installation.md) and [Network Configuration](/evolver/getting-started/configuring-computer-and-networking.md) should be complete.
{% endhint %}

## About

In order to appropriately interpret data from and actuate physical elements connected to the eVOLVER framework, a relationship must be established between the electrical signals generated by attached sensors and the physical phenomenon they are measuring. This is done by manually measuring values on a controlled set of data via some gold-standard assay or measurement device and comparing these data to the eVOLVER generated data. By doing this across a range of different values, a function can be fit to this relationship to allow eVOLVER to interpret data accurately.

For more information see [here](/evolver/software/dpu/calibration.md).

## Procedure

Calibrations should proceed in the following order:

1. [Temperature Calibration](/evolver/getting-started/calibrations/temperature-calibration.md)
2. [Optical Density Calibration](/evolver/getting-started/calibrations/optical-density-calibration.md)
3. [Pump Calibration](/evolver/getting-started/calibrations/pump-calibration.md)

{% hint style="warning" %}
If the GUI fails to calibrate you can try to use [calibrate.py](/evolver/getting-started/calibrations/manual-calibration-calibrate.py.md) manually.
{% endhint %}