# Use the GUI to Control Parameters
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Before doing using the GUI, all [Software Installation](https://khalil-lab.gitbook.io/evolver/getting-started/software-installation) and [Network Configuration](https://khalil-lab.gitbook.io/evolver/getting-started/configuring-computer-and-networking) should be complete.
Also, make sure to [calibrate](https://khalil-lab.gitbook.io/evolver/getting-started/calibrations) parameters before using them.
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Also Refer to the [JoVE ](https://www.jove.com/t/59652/designing-automated-high-throughput-continuous-cell-growth)paper for a video of using the GUI and the GUI Experiment Start [Guide](https://khalil-lab.gitbook.io/evolver/experiments/starting-an-experiment/gui-start-guide).
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Turn on the eVOLVER `5V` power supply by flipping the associated switch. *IMPORTANT: Wait for 5 seconds before turning on the `12V` power supply.*
Ensure the correct eVOLVER is selected by consulting the upper right hand corner of the GUI. It will display the eVOLVER name (default is evolver-darwin), followed by the IP address in parentheses. This will be the IP address you assigned it when setting up the router connecting the DPU to the vial platform.
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**If connected**, the **indicator dot** next to the name should be **green**. If this dot is **red**, you may need to troubleshoot the eVOLVER vial platform connection to the DPU. Consult the eVOLVER DPU [Installation](https://khalil-lab.gitbook.io/evolver/getting-started/software-installation/dpu-installation) and [Networking Setup](https://khalil-lab.gitbook.io/evolver/getting-started/configuring-computer-and-networking) for details on setting up and troubleshooting this connection. Otherwise consult the [forum](https://www.evolver.bio/) or post a question.
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In the `PARAMETER` box, ensure the correct calibrations are selected for Temp, OD, and the pumps. Click the box for each to bring up a list of available calibrations for that parameter.
Select all vials by tapping `Select All` in the bottom right corner of the screen. The vial circles on the right side of the display should become **orange**.
You can also click and drag to select as many vials as you want.
Tap the `NEXT →` on the box that says parameter to get to the `Stir` tab. Tap the `-` button or drag the slider to a Stir of 8. Press the `Stir: 8` button to alter the stirring of all vials.
Tap the `NEXT →` on the box that says parameter to get to the`Temperature` tab. Tap the `-` button or drag the slider until the middle button says `37.0 °C` and tap the button. The `EXECUTED COMMANDS` box should state that a stir and then temp command was sent to all vials.
## Pumps
Refer to the [JoVE](https://www.jove.com/v/59652/designing-automated-high-throughput-continuous-cell-growth) video for how to use pumps and sterilize fluidic lines
