# Use the GUI to Control Parameters

{% hint style="warning" %}
Before doing using the GUI, all [Software Installation](/evolver/getting-started/software-installation.md) and [Network Configuration](/evolver/getting-started/configuring-computer-and-networking.md) should be complete.

Also, make sure to [calibrate](/evolver/getting-started/calibrations.md) parameters before using them.
{% endhint %}

{% hint style="info" %}
Also Refer to the [JoVE ](https://www.jove.com/t/59652/designing-automated-high-throughput-continuous-cell-growth)paper for a video of using the GUI and the GUI Experiment Start [Guide](/evolver/experiments/starting-an-experiment/gui-start-guide.md).
{% endhint %}

Turn on the eVOLVER `5V` power supply by flipping the associated switch. *IMPORTANT: Wait for 5 seconds before turning on the `12V` power supply.*

Ensure the correct eVOLVER is selected by consulting the upper right hand corner of the GUI. It will display the eVOLVER name (default is evolver-darwin), followed by the IP address in parentheses. This will be the IP address you assigned it when setting up the router connecting the DPU to the vial platform.

{% hint style="info" %}
**If connected**, the **indicator dot** next to the name should be **green**. If this dot is **red**, you may need to troubleshoot the eVOLVER vial platform connection to the DPU. Consult the eVOLVER DPU [Installation](/evolver/getting-started/software-installation/dpu-installation.md) and [Networking Setup](/evolver/getting-started/configuring-computer-and-networking.md) for details on setting up and troubleshooting this connection. Otherwise consult the [forum](https://www.evolver.bio/) or post a question.
{% endhint %}

![](/files/w3eJwgwMQ3LxFz8HDFEX)

In the `PARAMETER` box, ensure the correct calibrations are selected for Temp, OD, and the pumps. Click the box for each to bring up a list of available calibrations for that parameter.

<figure><img src="/files/qFno5By0t30HZAOUwVJi" alt=""><figcaption></figcaption></figure>

Select all vials by tapping `Select All` in the bottom right corner of the screen. The vial circles on the right side of the display should become **orange**.

You can also click and drag to select as many vials as you want.

Tap the `NEXT →` on the box that says parameter to get to the `Stir` tab. Tap the `-` button or drag the slider to a Stir of 8. Press the `Stir: 8` button to alter the stirring of all vials.

<figure><img src="/files/nwlFvMYqIrd87pppC2Kd" alt=""><figcaption></figcaption></figure>

Tap the `NEXT →` on the box that says parameter to get to the`Temperature` tab. Tap the `-` button or drag the slider until the middle button says `37.0 °C` and tap the button. The `EXECUTED COMMANDS` box should state that a stir and then temp command was sent to all vials.

<figure><img src="/files/BiuIGoEf5vaiTtRdme5y" alt=""><figcaption></figcaption></figure>

## Pumps

Refer to the [JoVE](https://www.jove.com/v/59652/designing-automated-high-throughput-continuous-cell-growth) video for how to use pumps and sterilize fluidic lines

<figure><img src="/files/pqN5km08f0UJWOVuXLAc" alt=""><figcaption></figcaption></figure>


---

# Agent Instructions: Querying This Documentation

If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.

Perform an HTTP GET request on the current page URL with the `ask` query parameter:

```
GET https://khalil-lab.gitbook.io/evolver/guides/use-the-gui-to-control-parameters.md?ask=<question>
```

The question should be specific, self-contained, and written in natural language.
The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.

Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.