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eVOLVER
  • eVOLVER Documentation Wiki
  • General
    • About Us
    • eVOLVER Community
      • Code of Conduct
  • Getting Started
    • Buying eVOLVER
    • Part Sourcing
    • Unboxing and Setting Up
    • Software Installation
      • DPU Installation
      • Electron App (GUI) Installation
    • Configuring Computer and Networking
      • Router Setup
    • Calibrations
      • Temperature Calibration
      • Optical Density Calibration
      • Pump Calibration
      • Manual Calibration - calibrate.py
  • Upgrade Base eVOLVER Hardware
  • Experiments
    • Starting an Experiment
      • Carboy Media Prep
      • Preparing Vials
      • Setup Waste Carboy
      • Sterilizing Lines
      • Loading Vials and Setting Initial Conditions
      • OD Blank
      • GUI Start Guide
      • Command Line Start Guide
      • Cleaning Up After Experiment
    • Growth Curve
    • Chemostat
    • Turbidostat
    • FAQs
    • Tips and Tricks
  • Guides
    • Use the GUI to Control Parameters
    • Building a Smart Sleeve
    • Making media bottles and splitters
    • Emergency Efflux
    • View the Server Log and Restart Server
    • Updating the eVOLVER Server
    • Change Your conf.yml File
    • Arduino Software Installation
    • Raspberry Pi Configuration
    • Command Line Usage
    • Millifluidics Guides
      • Designing Millifluidics Using Eagle
      • Constructing Laser Cut Millifluidics
      • Constructing Millifluidics via SLA Printing
      • Calibrating IPPs
      • Operating Millifluidics
  • Troubleshooting
    • Troubleshooting Overview
    • Experiment Troubleshooting
      • Contamination
      • Vial Overflow, Pump Failure, and Spills
      • Tubing and Connector Blockage and Bursting
    • Optical Density (OD) Readings
    • Vial Troubleshooting
      • Replacing Photodiodes and LEDs
      • Heating Element
      • Stirring
    • Server Troubleshooting
    • Vial Platform Troubleshooting
      • Motherboard Troubleshooting/Replacement
      • 12V Power Supply Troubleshooting/Replacement
    • GUI Troubleshooting
    • eVOLVER Maintenance
  • Hardware
    • Overview of Hardware Architecture
    • Overview of Fluidics
      • Tubing and connectors
      • Peristaltic Pumps
      • Fluidics box
    • Overview of Millifluidics
      • IPPs (Integrated Peristaltic Pumps)
      • Pressure Regulator
    • Vial Caps
      • Universal Vial Cap Construction Guide (Luer Connectors Only)
      • 5 and 7 Port Nylon Tubing Caps Construction Protocol
    • Smart Sleeve
      • Vial Board
      • 🌪️Stirring
      • Temperature
      • Optical Density
        • OD90 vs OD135
    • Motherboard Layout and Circuitry
      • 🌡️Arduino
      • Sensor/Actuator Board Slots
      • Pulse Width Modulation (PWM) Boards
      • Analog-to-Digital Converter (ADC) Boards
    • Raspberry Pi
    • Chassis
    • Light Blocker / Splash Guard
    • Known Issues
  • Software
    • Overview of Software Architecture
    • DPU
      • Calibration
      • custom_script.py
      • Experiment Data Files
      • eVOLVER.py
    • Arduino
    • Server (Raspberry Pi)
      • Calibration Files
      • Configuration Files (conf.yml)
    • Known Issues
  • Extensions
    • Adding A New Experimental Parameter
      • Power Supply
      • Specific Applications
      • Custom Calibration Code
    • Custom Experiments
      • ePACE
        • ATTiny1634 Writing
        • LUX Board Troubleshooting
      • Morbidostat
      • Extractor Column
        • Extractor Volume Maintenance
        • Experiment Start
          • Sterilizing Extractor Fluidics
          • Setting up your experiment
          • Using the extractor script
        • Extractor Analysis
        • Troubleshooting
        • Example protocols
      • Phototroph Growth
        • Setup Phototroph eVOLVER
        • Photo-eVOLVER Smart Sleeves
          • Photo-eVOLVER Smart Sleeve Construction Guide
        • Experiment Guide
    • Custom Fluidics
      • Adding a Third Pump Rack
      • Bubblers / In-Vial Aeration
        • Bubbler Construction Protocol
        • Bubbler Cleaning Protocol
      • Running the slow pumps
    • min-eVOLVER
      • About
      • min-eVOLVER Construction
        • Parts
        • Construction Protocol
      • Fluidics Setup
      • Software Installation and Startup
      • send_command.py
      • Calibrations
      • Starting an Experiment
      • ePACE with min-eVOLVER
        • [v1.1] ePACE with min-eVOLVER
      • Troubleshooting
    • Interfacing with Other Systems
  • Contributing
    • Guidelines for Contribution
    • Reporting a Bug / Hardware Failure
    • Documentation
      • Making a Forum Post
      • How to Edit the Wiki
    • Software Development
    • Hardware Development
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On this page
  • 1) First Unboxing the System
  • 2) Set up power and ethernet for the vial platform
  • 3) Plug in and screw on Smart Sleeves
  • 4) Setup pump rack
  • 5) Plug in Power + Communications to Fluidics Platform
  • 6) Attach Pump Arrays to the Fluidics Box
  • 7) Label Smart Sleeves with Appropriate Label

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  1. Getting Started

Unboxing and Setting Up

PreviousPart SourcingNextSoftware Installation

Last updated 1 year ago

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If you have any questions about this process, feel free to reach out on the !

Congrats on getting your eVOLVER and supporting our open-sourced community. We rely on support from you guys to help sustain this ecosystem so we can let everyone do the experiments that they want to! In this tutorial, we are going to teach you how to put this system together:

If you ordered the standard kit, this is the tutorial for you! The kit contains the following major components:

  • Vial Platform

  • 16 Smart Sleeves

  • 2x Pump Arrays (total 32 pumps)

  • Fluidic Box to for control of 32 Pumps (can expand to 48 w/ additional hardware)

1) First Unboxing the System

There are several components with protective foam. Insure all the components were delivered intact.

16 Smart Sleeves and a Box of Accessories

Pump Array and Fluidics Box

Vial Platform

2) Set up power and ethernet for the vial platform

The Vial Platform is the unit on which all the Smart Sleeves will be screwed onto. This system contains the eVOLVER Motherboard, Raspberry Pi, and Power Modules that control the Smart Sleeves. Parts used to setup the Vial Platform should be labeled with a black dot.

Contents of the Vial Platform Components Bag:

3) Plug in and screw on Smart Sleeves

If the system turns ON properly, turn off the eVOLVER and start fastening the Smart Sleeves. Use the 4-40 screws to fasten down each Smart Sleeve (2 screws/ sleeve). Vial 0 is in the front left (closest to the touch screen) with vials 1, 2, and 3 on the right of vial 0, in that order. Vial 15 is in the back right (farthest from the touch screen).

4) Setup pump rack

The Fluidics Platform is a separate module than the Vial Platform, typically is placed next to the Vial Platform. For this next part, we are setting up a pump rack that goes on top of the Fluidics Platform.

First we want to set up the components for the pump rack. The parts for this is located in the Fluidic Box Components Bag (Red Dot).

We want to fasten (w/ M6 screws) in aluminum extrusion into 3D printed part for the front pieces first. The front piece are the ones with the longer feet. See below:

Next, insert back piece. Make sure the two feet are offset in the same direction. Do not screw the screws in yet. We will screw those in when we mount everything together with the acrylic piece.

Repeat for the other side.

Next, line up the acrylic piece to the back holes and fasten together with M6 screws.

Screw in 10-32 screws to the feet of the mounts to fasten the pump rack on top of the Fluidics Box. Repeat to fasten all four mounts

5) Plug in Power + Communications to Fluidics Platform

Next, let’s setup the fluidics box. The fluidic box requires several connections:

  • Two Power Entry Cables (one for 12V Power, other for 5V)

  • Serial Communication Cable (To communicate with Raspberry Pi in Vial Platform)

Power the 5V power in the Fluidic Box from the USB port of the Raspberry Pi.

Connect Serial Communication Cable between the Vial Platform and the Fluidics Platform.

6) Attach Pump Arrays to the Fluidics Box

The fluidic box is meant to be flexible, to power whatever fluidic components you want. The standard setup assumes the following setup (back of fluidics box):

Attach ribbon cables to the appropriate slots on the PCB the pumps are mounted on.

Top down view of ribbon cable connections.

Finally, attach the tubing to the appropriate pumps.

7) Label Smart Sleeves with Appropriate Label

If your smart sleeves were delivered unassembled use assembly guide.

First, plug in Power and the Ethernet Connection to the system and ensure all the components turn ON properly (e.g. the touch screen turns ON). For more information on how to configure the router, please read the .

The Ethernet should plug into your router, more information about assigning a static IP .

Labeling the vials with the appropriate color coded labels help minimize human error (I just print it and tape it on the vial). You can download a PDF of the file . The front left position (row closest to touchscreen) is vial 0.

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