# Morbidostat Morbidostat algorithms have been developed that gradually increase the selection pressure of an evolving culture, typically based on measured growth rate23. Previously, this algorithm has been implemented with two media inputs (+ and - drug), requiring three peristaltic pumps per culture (w/ efflux pump). In a 16-vial eVOLVER unit, this setup can easily be implemented by (1) controlling 48 pumps with the auxiliary board or (2) using multiplexed fluidics with the millifluidic devices. The prior being simpler to implement for 2 media inputs and the latter letting one scale to >2 inputs. As currently designed, the auxiliary board can control up to 48 fluidic elements (pumps/ solenoids). To run morbidostat mode, one would need to modify the Python code to the desired growth algorithm (e.g. control rate of drug increase, growth rate threshold to trigger the drug input). --- # Agent Instructions: Querying This Documentation If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question. Perform an HTTP GET request on the current page URL with the `ask` query parameter: ``` GET https://khalil-lab.gitbook.io/evolver/extensions/custom-experiments/morbidostat.md?ask= ``` The question should be specific, self-contained, and written in natural language. The response will contain a direct answer to the question and relevant excerpts and sources from the documentation. Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.