> For the complete documentation index, see [llms.txt](https://khalil-lab.gitbook.io/evolver/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://khalil-lab.gitbook.io/evolver/hardware/known-issues.md). # Known Issues {% hint style="info" %} Please report any issues you come across in the appropriate [Github repository](https://github.com/FYNCH-BIO/) and consider making a post on the [forum](https://www.evolver.bio/)! {% endhint %} ## Vials OD is dependent on depth of LED in vial * Characterized on this forum post * Needs to be pushed to users OD is dependent on vial position * Notched vial caps help align * Brandon has designed vial aligners * It is generally an unwritten rule to not move vials after experiment start, but this is sometimes impossible Stirring affects OD * Tuning stirring too high can cause stir bars to chitter (stop turning and move around) * Because the stirred media forms a spout in the middle * Therefore inconsistent stirring can means more noise in OD reading * Also, less stirring = less optimal growth ## Fluidics Pump heads stop working * Causes overflows, poor reliability * Fixed by new pumps * Almost all evolvers have old pumps ## eVOLVER Base New eVOLVER base susceptible to fluid damage * Vial overflows or spills get into chassis via ribbon cable ports, then short motherboard * Drainage system drips down the back of the eV and into chassis * Overflows finally leak onto bench and floor requiring serious cleanup Old eVOLVER base susceptible to fluid damage * Fluids from vials drip onto pumps, destroying them --- # Agent Instructions This documentation is published with GitBook. GitBook is the documentation platform designed so that both humans and AI agents can read, navigate, and reason over technical content effectively. Learn more at gitbook.com. ## Querying This Documentation If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question. Perform an HTTP GET request on the current page URL with the `ask` query parameter, and the optional `goal` query parameter: ``` GET https://khalil-lab.gitbook.io/evolver/hardware/known-issues.md?ask=&goal= ``` `ask` is the immediate question: it should be specific, self-contained, and written in natural language. `goal` is optional and describes the broader end goal you are ultimately trying to accomplish on behalf of the user. GitBook uses it to tailor the answer towards what is most useful for that goal. The response will contain a direct answer to the question and relevant excerpts and sources from the documentation. Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.