Known Issues

Please report any issues you come across in the appropriate Github repository and consider making a post on the forum!

Vials

OD is dependent on depth of LED in vial

• Characterized on this forum post

• Needs to be pushed to users

OD is dependent on vial position

• Notched vial caps help align

• Brandon has designed vial aligners

• It is generally an unwritten rule to not move vials after experiment start, but this is sometimes impossible

Stirring affects OD

• Tuning stirring too high can cause stir bars to chitter (stop turning and move around)

• Because the stirred media forms a spout in the middle

• Therefore inconsistent stirring can means more noise in OD reading

• Also, less stirring = less optimal growth

Fluidics

Pump heads stop working

• Causes overflows, poor reliability

• Fixed by new pumps

• Almost all evolvers have old pumps

eVOLVER Base

New eVOLVER base susceptible to fluid damage

• Vial overflows or spills get into chassis via ribbon cable ports, then short motherboard

• Drainage system drips down the back of the eV and into chassis

• Overflows finally leak onto bench and floor requiring serious cleanup

Old eVOLVER base susceptible to fluid damage

• Fluids from vials drip onto pumps, destroying them