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Known Issues

Please report any issues you come across in the appropriate Github repository and consider making a post on the forum!

Vials

OD is dependent on depth of LED in vial
  • Characterized on this forum post
  • Needs to be pushed to users
OD is dependent on vial position
  • Notched vial caps help align
  • Brandon has designed vial aligners
  • It is generally an unwritten rule to not move vials after experiment start, but this is sometimes impossible
Stirring affects OD
  • Tuning stirring too high can cause stir bars to chitter (stop turning and move around)
  • Because the stirred media forms a spout in the middle
  • Therefore inconsistent stirring can means more noise in OD reading
  • Also, less stirring = less optimal growth

Fluidics

Pump heads stop working
  • Causes overflows, poor reliability
  • Fixed by new pumps
  • Almost all evolvers have old pumps

eVOLVER Base

New eVOLVER base susceptible to fluid damage
  • Vial overflows or spills get into chassis via ribbon cable ports, then short motherboard
  • Drainage system drips down the back of the eV and into chassis
  • Overflows finally leak onto bench and floor requiring serious cleanup
Old eVOLVER base susceptible to fluid damage
  • Fluids from vials drip onto pumps, destroying them