Manual Calibration - calibrate.py

You can use manual calibration to view past calibrations after they are done. This can be useful for troubleshooting if a vial is not behaving as expected.

About

The GUI will run calibrations automatically upon completion of the calibration protocols. However, you can still manually run a calibration if you would like to change calibration settings. Can be useful if your GUI calibration fails for some reason.

Located in /dpu/calibration/.

Requirements

1. You have run calibration and logged raw values already

2. You are in your DPU virtual environment in command line. This is set up when you install the DPU.

Commands

List raw calibration files on the eVOLVER

Mac

python3 calibration/calibrate.py -a <ip_address> -g

For Windows, use py instead of python for all commands.

Calibrate Temperature

python3 calibration/calibrate.py -a <ip_address> -n <file_name> -t linear -f <name_after_fit> -p temp

Calibrate OD

OD135

python3 calibration/calibrate.py -a <ip_address> -n <file_name> -t sigmoid -f <name_after_fit> -p od_135

OD90 (Check to ensure mode is configured properly)

python3 calibration/calibrate.py -a <ip_address> -n <file_name> -t sigmoid -f <name_after_fit> -p od_90

3D FIT (Check to ensure mode is configured properly)

python3 calibration/calibrate.py -a <ip_address> -n <file_name> -t 3d -f <name_after_fit> -p od_90,od_135

Example Procedure to Plot OD90 After Calibration

1. Navigate to the dpu environment in terminal

   1. cd <your_dpu_address>

2. Put in the following command to get the exact name of your calibration file

   1. python3 calibration/calibrate.py -a <ip_address> -g

3. Do the following command, replacing ip_address, file_name, name_after_fit:

   1. python3 calibration/calibrate.py -a <ip_address> -n <file_name> -t sigmoid -f <name_after_fit> -p od_90

4. Close out of the python file and say no to updating the eVOLVER with new calibration