> For the complete documentation index, see [llms.txt](https://khalil-lab.gitbook.io/evolver/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://khalil-lab.gitbook.io/evolver/experiments/starting-an-experiment/setup-waste-carboy.md).

# Setup Waste Carboy

1. Place waste lines into carboy
2. We use a small stand to bring the carboy closer to the eVOLVER
3. Have an extra carboy ready to swap in when the first gets full
4. (Optional) You can fill 10% of the carboy with bleach
   1. Or bleach after depending on whether you hate the smell bleach or your culture more
5. (Optional) Shrink [heat shrink tubing](https://www.mcmaster.com/7132K519-7132K78/) onto the waste lines to make them easier to deal with
6. (Optional) Drill a hole through the carboy cap to make the opening to the carboy smaller (less smelly)
7. (Optional) Parafilm the opening to the carboy to make it less smelly

{% hint style="danger" %}
If you intend to leave overnight or for a weekend, make sure that the carboy has *more than enough* room in it for the maximum volume you will efflux. Consider using two carboys.
{% endhint %}

<figure><img src="/files/zQqNd87xRw6mGOCnMcrW" alt=""><figcaption><p>Waste carboy with black heat shrink over the waste lines and hole in carboy cap.</p></figcaption></figure>