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eVOLVER
  • eVOLVER Documentation Wiki
  • General
    • About Us
    • eVOLVER Community
      • Code of Conduct
  • Getting Started
    • Buying eVOLVER
    • Part Sourcing
    • Unboxing and Setting Up
    • Software Installation
      • DPU Installation
      • Electron App (GUI) Installation
    • Configuring Computer and Networking
      • Router Setup
    • Calibrations
      • Temperature Calibration
      • Optical Density Calibration
      • Pump Calibration
      • Manual Calibration - calibrate.py
  • Upgrade Base eVOLVER Hardware
  • Experiments
    • Starting an Experiment
      • Carboy Media Prep
      • Preparing Vials
      • Setup Waste Carboy
      • Sterilizing Lines
      • Loading Vials and Setting Initial Conditions
      • OD Blank
      • GUI Start Guide
      • Command Line Start Guide
      • Cleaning Up After Experiment
    • Growth Curve
    • Chemostat
    • Turbidostat
    • FAQs
    • Tips and Tricks
  • Guides
    • Use the GUI to Control Parameters
    • Building a Smart Sleeve
    • Making media bottles and splitters
    • Emergency Efflux
    • View the Server Log and Restart Server
    • Updating the eVOLVER Server
    • Change Your conf.yml File
    • Arduino Software Installation
    • Raspberry Pi Configuration
    • Command Line Usage
    • Millifluidics Guides
      • Designing Millifluidics Using Eagle
      • Constructing Laser Cut Millifluidics
      • Constructing Millifluidics via SLA Printing
      • Calibrating IPPs
      • Operating Millifluidics
  • Troubleshooting
    • Troubleshooting Overview
    • Experiment Troubleshooting
      • Contamination
      • Vial Overflow, Pump Failure, and Spills
      • Tubing and Connector Blockage and Bursting
    • Optical Density (OD) Readings
    • Vial Troubleshooting
      • Replacing Photodiodes and LEDs
      • Heating Element
      • Stirring
    • Server Troubleshooting
    • Vial Platform Troubleshooting
      • Motherboard Troubleshooting/Replacement
      • 12V Power Supply Troubleshooting/Replacement
    • GUI Troubleshooting
    • eVOLVER Maintenance
  • Hardware
    • Overview of Hardware Architecture
    • Overview of Fluidics
      • Tubing and connectors
      • Peristaltic Pumps
      • Fluidics box
    • Overview of Millifluidics
      • IPPs (Integrated Peristaltic Pumps)
      • Pressure Regulator
    • Vial Caps
      • Universal Vial Cap Construction Guide (Luer Connectors Only)
      • 5 and 7 Port Nylon Tubing Caps Construction Protocol
    • Smart Sleeve
      • Vial Board
      • 🌪️Stirring
      • Temperature
      • Optical Density
        • OD90 vs OD135
    • Motherboard Layout and Circuitry
      • 🌡️Arduino
      • Sensor/Actuator Board Slots
      • Pulse Width Modulation (PWM) Boards
      • Analog-to-Digital Converter (ADC) Boards
      • RS485 Board
    • Raspberry Pi
    • Chassis
    • Light Blocker / Splash Guard
    • Known Issues
  • Software
    • Overview of Software Architecture
    • DPU
      • Calibration
      • custom_script.py
      • Experiment Data Files
      • eVOLVER.py
    • Arduino
    • Server (Raspberry Pi)
      • Calibration Files
      • Configuration Files (conf.yml)
    • Known Issues
  • Extensions
    • Adding A New Experimental Parameter
      • Power Supply
      • Specific Applications
      • Custom Calibration Code
    • Custom Experiments
      • ePACE
        • ATTiny1634 Writing
        • LUX Board Troubleshooting
      • Morbidostat
      • Extractor Column
        • Extractor Volume Maintenance
        • Experiment Start
          • Sterilizing Extractor Fluidics
          • Setting up your experiment
          • Using the extractor script
        • Extractor Analysis
        • Troubleshooting
        • Example protocols
      • Phototroph Growth
        • Setup Phototroph eVOLVER
        • Photo-eVOLVER Smart Sleeves
          • Photo-eVOLVER Smart Sleeve Construction Guide
        • Experiment Guide
    • Custom Fluidics
      • Adding a Third Pump Rack
      • Bubblers / In-Vial Aeration
        • Bubbler Construction Protocol
        • Bubbler Cleaning Protocol
      • Running the slow pumps
    • min-eVOLVER
      • About
      • min-eVOLVER Construction
        • Parts
        • Construction Protocol
      • Fluidics Setup
      • Software Installation and Startup
      • send_command.py
      • Calibrations
      • Starting an Experiment
      • ePACE with min-eVOLVER
        • [v1.1] ePACE with min-eVOLVER
      • Troubleshooting
    • Interfacing with Other Systems
  • Contributing
    • Guidelines for Contribution
    • Reporting a Bug / Hardware Failure
    • Documentation
      • Making a Forum Post
      • How to Edit the Wiki
    • Software Development
    • Hardware Development
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On this page
  • Page under construction
  • Protocol
  • Control Layer (1/4" acrylic)
  • Flow Layer (1/8" acrylic)
  • Assembly
  • Subprotocols
  • Laser Cutter
  • Plasma Cleaning
  • Adhesive

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  1. Guides
  2. Millifluidics Guides

Constructing Laser Cut Millifluidics

Page under construction

Protocol

Control Layer (1/4" acrylic)

Cut outlines first

  • 0.1pt

  • 3 copies

Keep full stock in place + outline on computer

  • Acts as a frame for the device

  • Remove board from stock with tweezers

Add adhesive

Put adhesive covered outline back in place on stock

On the computer

  • Align control vector cuts (ports) to the outlines

  • Delete outlines

  • Print (3 copies for 1/4")

Keep board in place

Rastering control channel

  • Undo deletion of outline, delete previous vector cuts

  • Align raster outline to old outline + delete outline

  • Load control_channel 2 in advanced settings

    • 1200 dpi

    • Deep to get through adhesive

  • 1 copy => print

Remove board + stock from laser cutter

Flow Layer (1/8" acrylic)

Cut adhesive sheet

  • Copy/paste in design with scooped out region around bridge

  • Cut adhesive out first

  • This way we don't have to move the acrylic stock later to put adhesive on the laser cutter

  • Check adhesive stickiness

      • Remove corner from adhesive

      • One side will stay attached to adhesive, one will remove cleanly

  • Put adhesive sheet on laser cutter, with side that easily removes down

  • Focus to adhesive

  • Vector cut Full speed + 80 - 90% power

Cutting acrylic

  • Delete scoops, we just want the outlines

  • Print vector cut for outline

  • Refocus laser cutter to 1/8th inch + cut

Plasma clean acrylic

Put adhesive on

Rastering acrylic

  • Place device back in stock on laser cutter

  • Copy/paste raster of flow route

  • Remove outline

  • Raster cut

  • While rastering, start tapping control board

Assembly

Tap control board

  • 2 turns one way, little turn the other way

  • Use a countersink to remove burrs around the holes

    • A couple of turns

    • Both sides of board

    • Allows silicone to sit flush + barbs to insert easily

  • Blow out small bits of acrylic from threading using air before plasma treating

Remove backing from all acrylic (helps with alignment later)

Silicone

  • Sam from Densmore lab bought a big roll

  • Some in drawer under cutting station

  • Cut larger size than device

  • Peal one side of silicone

  • Plasma treat 30 seconds

Apply silicone to control board

  • Align and press onto control board

  • Take off plastic from other side of silicone

  • Wrap in paper towel

  • Put between aluminum plates in vice

  • Turn tight + one small more turn

  • Wait 10 minutes

Punch holes in silicone

  • Use biopsy punch (green on shelf above 3D printers) to make holes silicone for flow

  • Don't need to do the control

  • Put pressure and turn

Clean silicone + adhere to flow layer

  • Use piece of packing tape to remove dust

  • Plasma clean silicone 30 seconds

  • Align flow and control

    • Take special care to align IPP flow and control

    • Easier to align flow layer on top

  • Clamp full device in paper towel for 10 minutes

    • Avoid over tightening - this can overstretch the silicone layer

Put loctite on barbs + screw in to board

  • Barbs are in Dan's drawer

Subprotocols

Laser Cutter

Different colors of line produce different depth / intensity of cut

  • Use black for everything

Make all lines hairline outline

  • Otherwise it won't cut!

Open up a new page

  • Paste whatever you're cutting into this

  • This will be the correct size of bed

Printing

USB Bullshit

  • Open up printers / devices

  • Have to reconnect USB multiple times for it to be recognized

  1. Open up print

  2. Select correct printer you figured out

  3. Load the correct settings

  4. Set the correct copy number

    1. For millifluidics this is 2 for vector cutting (we have the double line problem)

    2. 1 for raster probably

  5. Press print

On the Laser Cutter

Make sure it's on / connected

Focus first using metal thing attached to laser

Run the job with lid open to double check it's doing what you hope

Run the job

Plasma Cleaning

Plasma treat surfaces (except adhesive)

  • Take off one side of acrylic backing

  • Vacuum knob arrow left

  • Metal valve tight, then quarter turn loose

  • Pump on + power on to remove air 1 minute

  • Then switch on upper right knob to high

    • Acrylic = 1 minute treatment

    • Silicone = 30 seconds treatment

  • Turn off pump and power

  • Slowly open black knob and let vacuum fill

Adhesive

Adhesive is in a box above 3D printers

Cut adhesive to larger size than acrylic

  • Remove adhesive backing and place face up on surface

  • Remove acrylic from plasma cleaner and firmly press clean side onto adhesive

  • Squeegee the bubbles out as much as you can (and away from where the channels will be)

  • Cut excess adhesive off with razor blade

PreviousDesigning Millifluidics Using EagleNextConstructing Millifluidics via SLA Printing

Last updated 2 years ago

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