Starting an Experiment
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You should have completed both and before proceeding to an experiment.
Ask about experiments in the relevant category on the .
Instead of the GUI, use send_command.py
The slow pumps (pink) will need to be run for >200 seconds to fully flush the lines
Use send_command.py
to fill the vials to appropriate volumes
Do not inoculate cells until after starting the experiment if you want accurate readings. There is innate variability in the OD of the vials (among other things) and we will blank to your vials during experiment start.
Use send_command.py
to set to correct temperature
A calibrated temperature command is in your calibration file temperature_calibration.xlsx
Temperature affects OD readings and we need a good initial OD to act as a "blank"
Allow min-eVOLVER to come to temperature
If running more than one min-eVOLVER at a time: you need a separate experiment folder and command line window for each min-eVOLVER (and different port number)
Start the experiment using the command:
python3 eVOLVER.py -i localhost:<your_min-eV_port>
python3 eVOLVER.py -i localhost:5555
Make sure that your eVOLVER computer is set to never sleep. If your computer falls asleep while you're running an experiment, you will not be collecting data during that time. See more info .
In a separate window from your server command line, follow the command line start .
the experiment
Data is in the