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Starting an Experiment
Highly recommended:
- 1.Read the main Experiment pages for helpful information not listed here and an example experimental workflow.
- 2.Run an experiment with water before doing so with cells, especially if you change the code
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- 3.
- 1.Instead of the GUI, use
send_command.py - 2.The slow pumps (pink) will need to be run for >200 seconds to fully flush the lines
You will have one command line window for each server and each experiment running. It can also be helpful to have a separate window for
send_commands.pyFor example for two min-eVOLVER experiments:
- 2 server windows (server environment)
- 2 experiment windows (
dpuenvironment) - 1
send_commands.pywindow (dpuenvironment)
An example fluidics layout for basic chemostat or turbidostat. If necessary, inducer can be programmed to be controlled via the low flow-rate pumps (not shown).
- 4.
- 1.Use
send_command.pyto fill the vials to appropriate volumes
Do not inoculate cells until after starting the experiment if you want accurate readings. There is innate variability in the OD of the vials (among other things) and we will blank to your vials during experiment start.
Vials set up for a basic chemostat or turbidostat.
- 5.Use
send_command.pyto set to correct temperature- 1.A calibrated temperature command is in your calibration file
temperature_calibration.xlsx - 2.Temperature affects OD readings and we need a good initial OD to act as a "blank"
- 6.Allow min-eVOLVER to come to temperature
Make sure that your eVOLVER computer is set to never sleep. If your computer falls asleep while you're running an experiment, you will not be collecting data during that time. See more info here.
- 1.If running more than one min-eVOLVER at a time: you need a separate experiment folder and command line window for each min-eVOLVER (and different port number)
- 2.Start the experiment using the command:
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python3 eVOLVER.py -i localhost:<your_min-eV_port> - 2.
python3 eVOLVER.py -i localhost:5555
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- 2.
Last modified 10d ago