OD Blank

Why

When we start an experiment, the script asks us whether we want to calibrate to blank.

OD is dependent on vial position, vial opacity, media, environmental light conditions, temperature, and more.

Therefore, we take the first OD measurement as a zero and subtract that out from every subsequent measurement.

Details

OD blank is only applied to the calculated OD from calibration, not the raw OD data. The blank is applied for each vial, it just subtracts it out from subsequent measurements for that vial

https://www.evolver.bio/t/where-or-how-is-od-blank-info-stored/182

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