Parts

Parts Spreadsheet

Below is a spreadsheet containing all parts required to make a min-eVOLVER.

1. Read the notes below the spreadsheet as well.

2. Don't miss the "Consumables / General" tab. These items also need to be acquired before running experiments.

The parts necessary for the min-eVOLVER main unit. Use the spreadsheet above to discern what each item is.

min-eVOLVER PCB

You can find the gerber files to order the PCB here.

If you are making a min-eVOLVER Control Board PCB from scratch you have two options (as of 11/20/2024):

1. Order an assembled PCB from PCBWay or another manufacturer

   1. You will need to also give them the centroid and bill of materials (BOM) files

   2. Give PCBway the zip file of all of the gerber files

2. Buy the parts needed from Digikey, order the PCB from PCBWay or another manufacturer, and solder the board yourself

   1. NOT recommended for anyone not already familiar with surface mount soldering.

   2. An interactive bill of materials (for part placement) can be found here. Download it and open it into your browser.

Pump PCB

Should be ordered from PCBWay or another manufacturer. You can find the gerber files here.

min-eVOLVER Case

Give Protocase the .x_t, .easm, and .step files found here.

12V Power Supply

Several 12V power supplies were tested and were selected for reliability and low noise. While most 12V power supplies should work well in the short term, long term usage may vary. Use a different power supply at your own risk.

Vial Cover

NOT AN OPTIONAL COMPONENT - Protects OD photodiodes from environmental light, which will completely change your OD calibrations. Also protects smart sleeves from spills and splashes.

3D print the latest vial cover to allow for more consistent OD readings. Choose the cheapest black FDM printing on hubs.com, shapeways.com, or a local 3D printing shop and give them the .stl file in the github repository.

eVOLVER Smart Sleeves

May be purchased from Fynch Bio. If interested in making your own see the main parts sheet and follow this assembly guide. This is not recommended for its added complication (you will need to machine the aluminum tube for example), but will save money.

Consumables / General

Listed in the "Consumables / General" sheet of the above spreadsheet

• Anything not listed as (Optional) is required for a full experiment.

• You need a thermometer for calibrating temperature - we suggest this one.

• If you plan to run multiple min-eVOLVERs from a computer, you will need to have a USB port available for each one. You can use a USB splitter dongle to accomplish this. Example here.