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The min-eVOLVER was originally conceived of as a minimal apparatus to conduct . Thus it has two bioreactors (eVOLVER ): one for a "host cell reservoir" and one for a "lagoon". It also has six pumps: four for bulk media in and out of the two Smart Sleeves and two for fine control over inducers into the lagoon.
However, base min-eVOLVER can be used for most continuous culture scenarios, such as turbidostat and chemostat. It can also be readily adapted to new experiments.
The software architecture of the min-eVOLVER is similar to the , with the exceptions that:
The Raspberry Pi server has been replaced with a server run on the connected laptop
A single Arduino SAMD21 actuates all functions and collects vial data through the min-eVOLVER board
Maximum current draw* = 2 Amps
Power supply voltage = 12V
Maximum power draw = 24W
*Maximum current draw was measured from a bench-top power supply with the heaters fully on, all pumps running, and stirring on. Instantaneous spikes in current could be higher when pumps and stirring start up
Especially if you plan to run multiple (>4) min-eVOLVERs off of one power strip, make sure you have a power strip that is rated for more than enough current. Most power strips are rated for 15A, but you should check.